Juq470 Verified -

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The reproducibility of pharmacological data relies heavily on the chemical integrity of small molecule probes. This technical note details the verification process for JUQ470 (CAS 74626-03-0), a potent and selective inhibitor of Rho-associated protein kinase (ROCK). Through a combination of Nuclear Magnetic Resonance (NMR) spectroscopy, Liquid Chromatography-Mass Spectrometry (LC-MS), and enantiomeric purity analysis, we confirm the structural identity and chemical purity of the JUQ470 batch. The verified compound demonstrates >99% chemical purity and consistent spectral data matching established literature, validating its suitability for mechanistic studies in cytoskeletal dynamics and smooth muscle contraction.